DESCRIPTION:
MEDNA M1 DNA polymerase (Taq) is a recombinant and thermostable
Taq polymerase (expressed from the Thermus aquaticus gene, BRENDA:
EC 2.7.7.7) expressed in E.coli. The enzyme is
robust in amplifying DNA fragments from 100 bp to 10 kb. By the addition of A
to the 3' end of the PCR product, the product can be cloned into TA vector
efficiently.
BACKGROUND:
Taq DNA Polymerase is a thermostable DNA polymerase that
possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease
activity. M1 DNA polymerase is used to amplify DNA in the polymerase chain
reaction (PCR). It can also be used in gene amplification, sequencing and
genotyping applications.
BIOACTIVITY:
Supplied with 10x Taq Pol II Reaction Buffer with or without 2.5
mM MgCl2. Concentration: 5 units/µL.
Unit definition: One unit will incorporate 10 nmols of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
