DESCRIPTION
This recombinant Human FcRn protein (the neonatal Fc receptor) is made from HEK-293 cells and fused with a polyhistidine tag at the C-terminus.
BIOACTIVITY
Binds to human IgG in a pH-dependent manner.
BACKGROUND
The neonatal Fc receptor (FcRn) is responsible for transporting maternal IgGs to fetus/newborns and
maintaining the homeostasis of IgGs in adults. FcRn resembles class I major histocompatibility complex in
structure, and is composed of a transmembrane heavy chain and an invariant β2 microglobulin. Changes in the
affinity of IgGs to to FcRn lead to changes in the half-life of engineered IgGs and Fc fusion proteins. Longer
half-life of therapeutic antibodies means lower dose and longer interval between administering. For some
diagnostic agents, including imaging or radio-labeled agents, a shorter half-life in circulation results in lower
non-specific binding and decreased side effects. Therefore, studying the interaction of FcRn and therapeutic
antibodies has direct clinical implications.
FORMULATION
0.2 μM filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.5
STORAGE
This protein should be stored at -80°C.
Stable at < -20°C for 3 months or at 4°- 7°C for 3 weeks.
Minimize repeated freeze-thaw cycles.
QUALITY CONTROL
Purity is greater than 95%.
