DESCRIPTION:
MEDNA BirA is
N-terminal tagged with FLAG-myc-His-MBP to provide the convenience to remove
the enzyme after biotinylation. MEDNA BirA enzyme is produced in E. coli
and purified to greater than 95% purity by affinity purification. The multiple
affinity tags and the high purity make this enzyme desirable over others available in the market.
BACKGROUND:
Biotin binds to avidin or streptavidin very tightly, and this interaction has been used in many applications. BirA biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to a lysine residue in a biotin-accepting polypeptide (such as the AviTag™ peptide). The protein is encoded by the birA gene.
