DESCRIPTION
MEDNA M1 DNA polymerase (Taq) is a recombinant and thermostable
Taq polymerase (expressed from the Thermus aquaticus gene, BRENDA:
EC 2.7.7.7) expressed in E.coli. The enzyme is
robust in amplifying DNA fragments from 100 bp to 10 kb. By the addition of A
to the 3' end of the PCR product, the product can be cloned into TA vector
efficiently.
BACKGROUND
Taq DNA Polymerase is a thermostable DNA polymerase that
possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease
activity. M1 DNA polymerase is used to amplify DNA in the polymerase chain
reaction (PCR). It can also be used in gene amplification, sequencing and
genotyping applications.
BIOACTIVITY
Supplied with 10x Taq Pol II Reaction Buffer with or without 2.5
mM MgCl2. Concentration: 5 units/µL.
Unit definition: One unit will incorporate 10 nmols of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
DOCUMENTS
