LafectineTM RU50 is a proprietary cationic lipid-based transfection reagent for mammalian cells. The ratio of LafectineTM RU50 to plasmid DNA is fixed at 50 µL LafectineTM RU50 for 1 µg of plasmid DNA. LafectineTM RU50 is formulated in a ready-to-use format - users simply add DNA and vortex the mix briefly. After a 15-minute incubation, the transfection mix can be added to cells. As judged by target protein production, the transfection efficiency of LafectineTM RU50 is consistently higher than that of similar products.