Activated HRP labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to Horseradish Peroxidase (HRP) with high sensitivity, high yield and low background. The technology is fully scalable from 10 µg to 1 g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.
Each kit contains the components necessary for at least five labeling reactions:
The following protocol is optimized for immunoglobulin labeling and gives a 4 fold HRP to IgG molar excess labeling ratio. The molar ratio of HRP to protein can be optimized for specific proteins or applications.
1. The starting concentration of the IgG solution must be at least 1.2 mg/mL before diluting it with Reaction Buffer to achieve optimal pH for labeling. A more dilute IgG solution can be buffer exchanged into Reaction Buffer and concentrated to achieve a final concentration of 1 mg/mL.
2.If less than 1 mg of IgG is to be labeled, use proportionally less activated HRP in the reaction.
2 - 8ºC