Production Introduction
Focurose 6FF is designed for component separation and moderate purification of biological macromolecules by removing small molecular impurities. It is suitable for applications such as virus particles, large protein molecules, supercoiled DNA, polysaccharides, and macromolecular complexes.
Features
Focurose 6FF Performance Parameters
|
Resin
|
Highly cross-linked 6% agarose |
|
Particle
size range |
45-165µm |
|
Average
particle size (D50) |
90±5µm |
|
Separation
range (globular proteins) |
10-4000 kDa |
|
pH
stability |
2-12 (long-term) 2-14 (short-term) |
|
Chemical
stability |
2M sodium hydroxide, 70% ethanol, 30%
isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea |
|
Linear
flow velocity (0.3 MPa) |
≥300 cm/h |
|
Maximum
pressure |
0.3 MPa |
|
Storage
solution |
20% ethanol |
|
Storage
conditions |
4℃-30℃ |
Frequently Asked Questions and Solutions
|
Issue |
Possible Causes |
Solutions |
|
Rapid increase
in chromatographic peak |
Overly tight
packing of the resin |
Repack the
column |
|
Slow or tailing
chromatographic peak |
Loose packing of
the resin |
Repack the
column |
|
Cracks or
dryness in the column bed |
Leakage or
introduction of large air bubbles |
Check for leaks
or bubbles in the tubing and repack the column if necessary |
|
Poor separation
efficiency |
1.
Inappropriate choice of
matrix |
Verify if the
selected matrix is suitable |
|
2.
Poor column performance |
Evaluate the
column efficiency |
|
|
3.
Oversampling |
Optimize the
optimal sampling volume |
|
|
4.
Excessive flow rate |
Optimize the
optimal flow rate |
|
|
Slow liquid flow |
1.
Protein or lipid
aggregation |
Clean the matrix
or filter membrane promptly |
|
2.
Protein precipitation
in the matrix |
Adjust the
composition of equilibration and elution buffers to maintain the stability of
the target substance and the binding efficiency of the matrix |
|
|
3.
Microbial growth in the
separation column |
Reagents used
must be filtered and degassed; samples must be centrifuged or filtered before
loading onto the column |
