Purification & Detection

Focurose 6FF

Catalog # Size Price Quantity
V6666 100 mL $208.00
V6667 250 mL $390.00
V6668 1 L $1,300.00
V6665 25 mL $78.00

Production Introduction

Focurose 6FF is designed for component separation and moderate purification of biological macromolecules by removing small molecular impurities. It is suitable for applications such as virus particles, large protein molecules, supercoiled DNA, polysaccharides, and macromolecular complexes.


  • High (physicochemical) stability, high flow rate (component separation), and high recovery rate (up to 95%)
  • Gentle elution conditions to preserve the biological activity and functionality of biological macromolecules
  • Easy to scale up
  • Easy to maintain 

Focurose 6FF Protocol

Focurose 6FF Performance Parameters


Highly cross-linked 6% agarose

Particle size range 


Average particle size (D50)


Separation range (globular proteins)

10-4000 kDa

pH stability

2-12 (long-term) 2-14 (short-term)

Chemical stability

2M sodium hydroxide, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea

Linear flow velocity (0.3 MPa)

≥300 cm/h

Maximum pressure

0.3 MPa

Storage solution

20% ethanol

Storage conditions


Frequently Asked Questions and Solutions


Possible Causes


Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if necessary

Poor separation efficiency

1.       Inappropriate choice of matrix

Verify if the selected matrix is suitable

2.       Poor column performance

Evaluate the column efficiency

3.       Oversampling

Optimize the optimal sampling volume

4.       Excessive flow rate

Optimize the optimal flow rate

Slow liquid flow

1.       Protein or lipid aggregation

Clean the matrix or filter membrane promptly

2.       Protein precipitation in the matrix

Adjust the composition of equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the matrix

3.       Microbial growth in the separation column

Reagents used must be filtered and degassed; samples must be centrifuged or filtered before loading onto the column