Protein Introduction
Focurose 4FF is suitable for component separation and moderate purification of biomacromolecules (removal of small molecule impurities), such as viral particles, large molecular proteins, recombinant hepatitis B surface antigen, polysaccharides, and macromolecular complexes.
Features
Focurose 4FF Performance Parameters
|
Resin
|
Highly cross-linked 4% agarose |
|
Particle
size range |
45-165µm |
|
Average
particle size (D50) |
90±5µm |
|
Separation
range (globular proteins) |
60-20000 kDa |
|
pH
stability |
2-12 (long-term) 2-14 (short-term) |
|
Chemical
stability |
2M sodium hydroxide, 70% ethanol, 30%
isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea |
|
Linear
flow velocity (0.3 MPa) |
≥250 cm/h |
|
Maximum
pressure |
0.3 MPa |
|
Storage
solution |
20% ethanol |
|
Storage
conditions |
4℃-30℃ |
Frequently Asked Questions and Solutions
|
Issue |
Possible Causes |
Solutions |
|
Rapid increase
in chromatographic peak |
Overly tight packing of the resin |
Repack the
column |
|
Slow or tailing chromatographic
peak |
Loose packing of the resin |
Repack the
column |
|
Cracks or
dryness in the column bed |
Leakage or introduction of large air bubbles |
Check for leaks
or bubbles in the tubing and repack the column if necessary |
|
Poor separation efficiency |
Inappropriate choice of matrix |
Verify if the
selected matrix is suitable |
|
Poor column performance |
Evaluate the
column efficiency |
|
|
Oversampling |
Optimize the
optimal sampling volume |
|
|
Excessive flow rate |
Optimize the
optimal flow rate |
|
|
Slow liquid flow |
Protein or lipid aggregation |
Clean the matrix
or filter membrane promptly |
|
Protein precipitation in the matrix |
Adjust the
composition of equilibration and elution buffers to maintain the stability of
the target substance and the binding efficiency of the matrix |
|
|
Microbial growth in the separation column |
Reagents used
must be filtered and degassed; samples must be centrifuged or filtered before
loading onto the column |
