Purification & Detection

Focurose 4FF

Catalog # Size Price Quantity
V4446 100 mL $192.00
V4447 250 mL $360.00
V4448 1 L $1,200.00
V4445 25 mL $72.00

Protein Introduction

Focurose 4FF is suitable for component separation and moderate purification of biomacromolecules (removal of small molecule impurities), such as viral particles, large molecular proteins, recombinant hepatitis B surface antigen, polysaccharides, and macromolecular complexes.


  • High (physiochemical) stability, high flow rate (component separation), and high recovery rate (up to 95%)
  • Mile elution conditions that preserve the biological activity and functionality of biomacromolecules
  • Easy to scale up
  • Easy to maintain

Focurose 4FF Performance Parameters


Highly cross-linked 4% agarose

Particle size range 


Average particle size (D50)


Separation range (globular proteins)

60-20000 kDa

pH stability

2-12 (long-term) 2-14 (short-term)

Chemical stability

2M sodium hydroxide, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea

Linear flow velocity (0.3 MPa)

≥250 cm/h

Maximum pressure

0.3 MPa

Storage solution

20% ethanol

Storage conditions


Frequently Asked Questions and Solutions


Possible Causes


Rapid increase in chromatographic peak

Overly tight packing of the resin

Repack the column

Slow or tailing chromatographic peak

Loose packing of the resin

Repack the column

Cracks or dryness in the column bed

Leakage or introduction of large air bubbles

Check for leaks or bubbles in the tubing and repack the column if necessary

Poor separation efficiency

Inappropriate choice of matrix

Verify if the selected matrix is suitable

Poor column performance

Evaluate the column efficiency


Optimize the optimal sampling volume

Excessive flow rate

Optimize the optimal flow rate

Slow liquid flow

Protein or lipid aggregation

Clean the matrix or filter membrane promptly

Protein precipitation in the matrix

Adjust the composition of equilibration and elution buffers to maintain the stability of the target substance and the binding efficiency of the matrix

Microbial growth in the separation column

Reagents used must be filtered and degassed; samples must be centrifuged or filtered before loading onto the column