Purification & Detection

Ni Focurose FF (IMAC)

Catalog # Size Price Quantity
V8045 25 mL $306.00
V8046 100 mL $816.00
V8047 250 mL $1,530.00
V8048 1 L $5,100.00

Product Introduction

Ni Focurose FF (IMAC) utilizes the interaction between Ni2+ and certain amino acids on the side chains of proteins (primarily histidine, cysteine, and tryptophan) for separation and purification. It is suitable for the separation and purification of His-tagged proteins and other biomolecules that interact with Ni2+ .


  • Fast and simple (one-step purification) ✓ Wide applicability and easy operation, suitable for gravity columns and prepacked columns (peristaltic pump or chromatography systems)
  • Multiple choices, can chelate various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use
  • Compared to Ni Focurose FF (IDA), Ni2+ detachment is low and it has broad reagent compatibility
  • Note: When chelating Ca2+, avoid using phosphate buffer (as it may form precipitates).

Ni Focurose FF (IMAC) Protocol

Ni Focurose FF (IMAC) Performance Parameters


Highly cross-linked 6% agarose

Particle size range 


Average particle size (D50)


Binding capacity

≥40 mg (His-tagged protein)/mL (resin)

Metal ion loading

16-23 μmol (Ni2+) / mL (resin)

pH stability

3-12 (long-term) 2-14 (short-term)

Chemical stability

Avoid using chelating agents (such as EDTA, EGTA) and reducing agents (such as DTT and DTE) in all commonly used aqueous solutions and buffers.

Linear flow velocity (0.3 MPa)

≥300 cm/h

Operating pressure


Storage solution

20% ethanol

Storage conditions


* Stability refers to the stability of the resin in the absence of chelated metal ions.