Purification & Detection

Ni Focurose FF (IMAC)

Catalog #	Size	Price
V8045	25 mL	$306.00
V8046	100 mL	$816.00
V8047	250 mL	$1,530.00
V8048	1 L	$5,100.00

Product Introduction

Ni Focurose FF (IMAC) utilizes the interaction between Ni2+ and certain amino acids on the side chains of proteins (primarily histidine, cysteine, and tryptophan) for separation and purification. It is suitable for the separation and purification of His-tagged proteins and other biomolecules that interact with Ni2+ .

Features

Fast and simple (one-step purification) ✓ Wide applicability and easy operation, suitable for gravity columns and prepacked columns (peristaltic pump or chromatography systems)
Multiple choices, can chelate various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use
Compared to Ni Focurose FF (IDA), Ni2+ detachment is low and it has broad reagent compatibility
Note: When chelating Ca2+, avoid using phosphate buffer (as it may form precipitates).

Ni Focurose FF (IMAC) Protocol

Ni Focurose FF (IMAC) Performance Parameters

Resin	Highly cross-linked 6% agarose
Particle size range	45-165µm
Average particle size (D50)	90±5µm
Binding capacity	≥40 mg (His-tagged protein)/mL (resin)
Metal ion loading	16-23 μmol (Ni²⁺) / mL (resin)
pH stability	3-12 (long-term) 2-14 (short-term)
Chemical stability	Avoid using chelating agents (such as EDTA, EGTA) and reducing agents (such as DTT and DTE) in all commonly used aqueous solutions and buffers.
Linear flow velocity (0.3 MPa)	≥300 cm/h
Operating pressure	≤0.3MPa
Storage solution	20% ethanol
Storage conditions	4℃-30℃

* Stability refers to the stability of the resin in the absence of chelated metal ions.

Ni Focurose FF (IMAC)

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