Enzymes & Accessories

M4 PNGase F

Catalog # Size Price Quantity
M3104 15,000 units $180.00
M3105 75,000 units $780.00


M4 PNGase F (EC: is used for deglycosylation of proteins. The highly pure enzyme of MEDNA is excellent for cleavage of N-linked glycan from glycoproteins. M4 PNGase F can be used for any application that requires the removal of N-linked glycans from proteins,  for example, studying the N-glycosylation of proteins of interest by mass spectrometry.



Glycosylation is a common post-translational modification with an important role in many cellular processes. It also affects the efficacy of biologics therapies. PNGase F is an effective tool to study the glycosylation status of proteins of interest. PNGase F (peptide-N-glycosidase F), also known as peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase, releases intact N-glycans by cleaving between the innermost GlcNAc of the N-glycan and an Asparagine (Asn) residue.


Maley et al., 1989, “Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases.” Analytical Biochemistry, 180(2) 195-204.

Elder, JH and S. Alexander, 1982, “endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins”, PNAS, 79(15): 4540-4.



100 mM HEPES pH 6.0. 0.2 µm filtered solution.



Long term storage at -80ºC.

Reconstituted protein solution can be stored at 2-8ºC for 2-7 days.

Aliquots of reconstituted protein solution is stable at <-20ºC for 3 months.



Purity: >95%.