M4 PNGase F (EC: 22.214.171.124) is used for deglycosylation of proteins. The highly pure enzyme of MEDNA is excellent for cleavage of N-linked glycan from glycoproteins. M4 PNGase F can be used for any application that requires the removal of N-linked glycans from proteins, for example, studying the N-glycosylation of proteins of interest by mass spectrometry.
Glycosylation is a common post-translational modification with an important role in many cellular processes. It also affects the efficacy of biologics therapies. PNGase F is an effective tool to study the glycosylation status of proteins of interest. PNGase F (peptide-N-glycosidase F), also known as peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase, releases intact N-glycans by cleaving between the innermost GlcNAc of the N-glycan and an Asparagine (Asn) residue.
Maley et al., 1989, “Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases.” Analytical Biochemistry, 180(2) 195-204.
Elder, JH and S. Alexander, 1982, “endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins”, PNAS, 79(15): 4540-4.
100 mM HEPES pH 6.0. 0.2 µm filtered solution.
Long term storage at -80ºC.
Reconstituted protein solution can be stored at 2-8ºC for 2-7 days.
Aliquots of reconstituted protein solution is stable at <-20ºC for 3 months.