M4 PNGase F (EC:
3.5.1.52) is used for deglycosylation of proteins. The highly pure enzyme of
MEDNA is excellent for cleavage of N-linked glycan from glycoproteins. M4
PNGase F can be used for any application that requires the removal of N-linked
glycans from proteins, for example, studying
the N-glycosylation of proteins of interest by mass spectrometry.
BACKGROUND:
Glycosylation is a common post-translational modification with an important role in many cellular processes.
It also affects the efficacy of biologics therapies. PNGase F is an effective
tool to study the glycosylation status of proteins of interest. PNGase F
(peptide-N-glycosidase F), also known as peptide-N4-(N-acetyl-β-glucosaminyl)
asparagine amidase, releases intact N-glycans by cleaving between the innermost
GlcNAc of the N-glycan and an Asparagine (Asn) residue.
REFERENCES:
Maley et al.,
1989, “Characterization of glycoproteins and their associated oligosaccharides
through the use of endoglycosidases.” Analytical Biochemistry, 180(2)
195-204.
Elder, JH and S. Alexander,
1982, “endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium
meningosepticum that cleaves both high-mannose and complex glycoproteins”, PNAS,
79(15): 4540-4.
FORMULATION:
100 mM HEPES pH 6.0.
0.2 µm filtered solution.
STORAGE:
Long term storage at -80ºC.
Reconstituted protein
solution can be stored at 2-8ºC for 2-7 days.
Aliquots of
reconstituted protein solution is stable at <-20ºC for 3 months.
QUALITY CONTROL:
Purity: >95%.
