DESCRIPTION
Streptavidin Agarose is prepared by conjugating streptavidin to heat stable, cross linked 4% agarose gel beads. To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm.
PRODUCT ADVANTAGES
The procedure we have developed for coupling streptavidin to agarose preserves the biotin binding activity of the streptavidin. Unlike cyanogen bromide coupling, our procedure does not produce conjugates which can bleached from the gel with common solutes such as Tris buffer. Our procedure also does not generate charged groups on the gel that can bind proteins nonspecifically.
This product can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays.
- Can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays.
- Prepared by conjugating streptavidin to heat stable, cross linked 4% agarose gel beads.
- To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm.
- Biotin binding activity of the streptavidin optimally preserved through proprietary coupling procedure.
- Streptavidin cannot be leached from gel with solutes such as Tris buffer.
- Low nonspecific binding to the gel.
- 1 mg streptavidin per mL of settled agarose beads.
- Supplied as a 1:1 suspension in buffer.
- Binding capacity is approximately 1 mole of biotinylated HRP per mole of streptavidin.
STORAGE
Store at 2°-8ºC.
