DESCRIPTION:
This recombinant Human IgG1 Fc protein is produced in and purified
from HEK293 cells. It is great to use as a negative control in binding assays
such as ELISA and flow cytometry that use antibodies containing the human IgG1
Fc domain as a constant region.
BACKGROUND:
Recombinant fusion proteins consisting of the extracellular
domain of immunoregulatory proteins and the constant (Fc) domain of
immunoglobulin G1 (IgG1) represent a growing class of human therapeutics.
Chimeric protein of Human antigen-targeting variable domain and Human IgG-Fc
domain can be purified using protein A chromatography. Our product, E1011, is
the Human IgG1 Fc domain control protein, purified using protein A-agarose (GE,
cat. # 17-5438). MW ~55 kDa.
FORMULATION:
100 mM HEPES, 0.2% sodium acetate, pH 6. 0.2 µm filtered solution.
STORAGE:
Long term storage at -80℃.
Stable at <-20℃ for 3 months or at
4-7℃ for 3 weeks.
Minimize repeated freeze-thaw cycles.
QUALITY CONTROL:
Purity: >95%.
