DESCRIPTION:
Zaire ebolavirus spike glycoprotein (NP_066246) (Ile33-Arg649) was expressed with the Flag-Myc-polyhistidine
tag at the N-terminus.
BIOACTIVITY:
Shown to bind to Human anti-GP Antibody (Catalog No. H1303)
BACKGROUND:
The fourth gene of the Ebola virus (EBOV) genome encodes a 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa
secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have
different C-terminal sequences. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2,
which are generated from the proteolytical process of the GP precursor by cellular furin during virus assembly. The
GP1 subunit contains a mucin domain and a receptor-binding domain (RBD). The GP2 subunit has a fusion
peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail.
Ebola GP Secreted Form is GP expressed with the Flag-Myc-polyhistidine tag at the N-terminus, and the removal
of the transmembrane domain and cytoplasmic tail.
FORMULATION:
0.2 μM filtered solution of 30 mM HEPES, 150 mM NaCl, pH 7.0
STORAGE:
Store under sterile conditions at -20°C to -80°C.
Samples are stable at -70°C for up to twelve months from date of receipt.
It is recommended that the protein be aliquoted for optimal storage.
Avoid repeated freeze-thaw cycles.
QUALITY CONTROL:
Endotoxin measures at <0.1 EU/ µg protein as determined by the LAL method.
