M5 DNA polymerase (pfu) is an engineered,
high-fidelity DNA polymerase from Pyrococcus furiosus recombinantly expressed
in E. coli. It is used for high fidelity applications due to its 3´→5´
exonuclease (proofreading) activity.
BACKGROUND:
Pfu DNA Polymerase is a thermostable enzyme that
has optimum activity at 75℃ in
the presence of magnesium. It has greater than 10x fidelity than Taq DNA
polymerase (Lundberg et al., 1991, Gene, 108(1):1-6).
FORMULATION:
20
mM Tris pH 7.9, 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1 mM DTT, 50%
glycerol. 0.2 µm filtered solution.
USAGE:
Conventional
Pfu Polymerase PCR ingredients, buffers, conditions and protocols will apply.
STORAGE:
Storage at -80ºC Reconstituted protein solution can be stored at 2-8ºC for 7-14 days. Avoid multiple freeze-thaw cycles.
QUALITY CONTROL:
Purity: ≥85%.
