M19 RNase A is used to remove RNA from DNA
plasmid preparations and protein samples. It is also used in RNA sequence
analysis and protection assays. M19 Rnase A is recombinantly expressed in and
purified from E. coli.
BACKGROUND:
M19
Ribonuclease A is an endoribonuclease that attacks at the 3’OHphosphate of a
pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give
pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
FORMULATION:
20
mM Tris pH 8.0. 0.2 µm filtered solution.
ACTIVITY/USAGE:
1,000
U/mL. Use on clarified lysate at a final concentration of 100 U/mL for 30 min
at room temperature.
APPLICATIONS:
Removal of RNA during plasmid DNA purification. Removal of unspecifically bound RNA. RNAse protection assays. Analysis RNA sequences. Hydrolysis of RNA in protein samples.
STORAGE:
-80 °C
QUALITY CONTROL:
˃95% purity by SDS densitometry.
